Eppendorf epMotion Liquid Handeling Workstations F.A.Q and Troubleshooting

epMotion Liquid Handling Workstations

How many deck positions do the various epMotion liquid handling workstations have?

The positions on the deck of the four epMotion models are as follows:

epMotion 5070: 4 positions
epMotion 5075 LH: 12 positions
epMotion 5075 VAC: 11 open positions and 1 vacuum station
epMotion 5075 MC: 9 open positions and an area for a Mastercycler

Various accessories and labware with a uniform area can be fixed in the positions on the deck. Each position satisfies the requirements of the SBS* Microplate Specifications for microplates. The following materials can be used: micro test plates (MTP), racks with pipette tips, racks for tubes, racks for tubs, Thermoblocks for Eppendorf micro test tubes, particular tubes, and PCR plates.

*SBS: Society for Biomolecular Screening

Can the epMotion workstations be controlled with a PC?

Not directly. Operation of the epMotion workstations is carried out using a Control Panel; a PC isn’t necessary. The Control Panel can be operated using the integrated keypad or the mouse. Programming of methods can be carried out either using the Control Panel or a PC (optional with epMotion Editor software). Method programs written on a PC can be transported to the Control Panel (and vice versa) with a MultiMediaCard™ (MMC). Printouts from method programs or from the log file can only be carried out using the PC.

The MMC also serves as a medium for saving method programs outside of the Control Panel or PC, as well as to update methods or software.

What purpose does the Optical Sensor serve?

Up to three different functions can be carried out with the Optical Sensor. First, the sensor can be used to check the number and type of tips currently found on the deck. Second, the labware used (plate, holder, and rack types) can be scanned in order to check whether the labware actually being used is positioned correctly and matches the labware defined in the method. Third, the Optical Sensor can determine the liquid level in tubes, reagent reservoirs, and even micro test plates.

What is the working volume range of the epMotion workstations?

The epMotion workstations can pipette or dispense 1 µl to 1,000 µl: The volume range is subdivided into three ranges (1 µl–50 µl, 20 µl–300 µl, and 40 µl–1,000 µl), which are segmented according to the individual dispensing tool

Eppendorf Centrifuge F.A.Q

How do I open the centrifuge lid in case of a power failure?
Eppendorf Microcentrifuge 5415 C 
Turn off the main power switch, unplug the centrifuge, and wait until the rotor stops spinning. Locate the pinhole on the left side of the housing, near the top right corner. Insert a paper clip and the lid will open.
Eppendorf Microcentrifuge 5415 D 
Turn off the main power switch, unplug the centrifuge, and wait until the rotor stops spinning. Locate the white plastic knob near the front right corner of the bottom base plate. The knob is attached to the emergency lid release mechanism by a string. Pull on the plastic knob while pushing down on the centrifuge lid and the lid will open.
Eppendorf Microcentrifuge 5415 R 
Turn off the main power switch, unplug the centrifuge, and wait until the rotor stops spinning. Remove the white cap near the front of the right side of the housing. Insert the rotor tool and turn clockwise while pushing down on the lid. The lid will open.
Eppendorf Microcentrifuge 5417 C and 5417 R 
Turn off the main power switch, unplug the centrifuge, and wait until the rotor stops spinning. Locate the plastic knob near the front left corner of the bottom base plate. The knob is attached to the emergency lid release mechanism by a string. Pull on the knob and the lid will open.
Eppendorf MiniSpin® and MiniSpin plus Personal Micro Centrifuges 
Turn off the main power switch, unplug the centrifuge, and wait until the rotor stops spinning. Insert a pen into the opening near the front right corner of the bottom plate of the centrifuge and move the disc counterclockwise until the lid opens.
Eppendorf Multipurpose Centrifuges 5804, 5804 R, 5810, and 5810 R 
Turn off the main power switch, unplug the centrifuge, and wait until the rotor stops spinning. Remove the cap located on the front housing, near the bottom center. Insert the rotor tool into the hole and turn counterclockwise until lid opens.
Eppendorf General Purpose Centrifuge 5702
Turn off the main power switch, unplug the centrifuge, and wait until the rotor stops spinning. Locate the plastic cap near the front right suction foot on the bottom base plate. The cap is attached to the emergency lid release mechanism by a string. Pull on the cap and the lid will open.
I have just received an Eppendorf Microcentrifuge 5417 C (or 5417 R) and a standard 30-place Fixed-angle Rotor. How do I install the rotor seal supplied with the rotor?

A seal has been installed by the manufacturer, therefore you do not need to install the spare seal provided.

I have an Eppendorf Multipurpose Centrifuge 5804 (5804 R, 5810, or 5810 R) and just changed my rotor from a Swing-bucket Rotor to a 6 x 85 ml Fixed-angle Rotor. Why does the display still indicate the maximum speed of the Swing-bucket Rotor? (In other words, the centrifuge is not recognizing the rotor.)

Eppendorf Multipurpose Centrifuges feature automatic rotor recognition. When you press Start, the centrifuge will ramp up to 200 rpm, display the new maximum speed, and then come to a stop. For manual rotor recognition, hold down the Start button and spin the rotor until the display changes to the appropriate maximum speed.

I just received a new Horizontal Drum Rotor or Deep-well Rotor for my Multipurpose Centrifuge 5804 (5804 R, 5810, or 5810 R). Why is my centrifuge not recognizing these new rotors?

The new rotors are compatible with software versions greater than 2.1. Check the software version by turning on the centrifuge and reading the display. New software EPROM’s are included with each new rotor and should be installed by an authorized Brinkmann service representative. Please contact our service department to schedule an appointment for installation of the new EPROM.

I just purchased an Eppendorf Microcentrifuge 5415 D and I did not receive a rotor tool.

The rotor tool is packed in the Styrofoam shipping insert.

I did not receive a rotor with my Eppendorf Microcentrifuge 5415 D.

For user convenience, Model 5415 D is sold either with or without rotor. The catalog numbers reflecting both options are listed below:

022621203 Microcentrifuge 5415 D, with 24-place 1.5–2.0 ml rotor
022621211 Microcentrifuge 5415 D, without rotor

Please check the catalog number of the version you’ve received and compare it to your purchase order. If you ordered catalog number 022621203 and did not receive a rotor, please contact our Customer Support department.

How often should I lubricate my centrifuge motor shaft?

Every time you change or install a new rotor.

Why can’t my Refrigerated Multipurpose Centrifuge 5804 R (or 5810 R) maintain 4°C at maximum speed?

The Multipurpose Centrifuges 5804 R and 5810 R can maintain 4°C at maximum speed with all rotors except the 6 x 85 ml Fixed-angle Rotor (022627207). If 4°C cannot be maintained at maximum speed with rotors other than the 6 x 85 ml Fixed-angle Rotor, please contact Brinkmann Instruments Services.

The following specifications apply to the 6 x 85 ml Fixed-angle Rotor only:

  • Models 5804 R and 5810 R can maintain 4°C with this rotor at 10,500 rpm
  • Model 5804 R can maintain 6°C at maximum speed
  • Model 5810 R can maintain 12.5°C at maximum speed
Why can’t my Refrigerated Centrifuge Model 5417 R, 5804 R, or 5810 R maintain 4°C overnight?

The refrigeration system of Eppendorf Refrigerated Centrifuge Models 5417 R, 5804 R, and 5810 R is designed to shutdown after six hours from the last time of use. This feature conserves energy, minimizes condensation buildup, and prolongs the lifetime of the centrifuge. Although it is not recommended, this feature may be switched off. Please contact Brinkmann Instruments Services for instructions.

Can I spin different tube sizes in my Multipurpose Centrifuge Swing-bucket Rotor?

Yes. Tubes of different volumes can be loaded in adjacent buckets, but not in opposite buckets. Please refer to the Operation Manual for loading Swing-bucket Rotors.

What is the warranty of my centrifuge?

Models 5415 D, 5415 R, 5417 C, 5417 R, 5702, 5702 R, 5804, 5804 R, 5810, 5810 R, MiniSpin, and MiniSpin plus all feature a two-year warranty. Models 5415 C and 5410 have a one-year warranty.

How should I clean my Centrifuge Rotor?

Rotors should be cleaned with neutral cleaning agents such as EXTRAN® neutral or isopropanol. Use neutral decontaminants such as RAD-Con and Radiacwash for radioactive decontamination. Do not use alkaline-cleaning agents such as Count-off.

I want to convert from RPM to RCF. What is the radius of my Model 5417C/R 30-place Fixed-angle Rotor?

Both models 5417C and 5417R can convert from RPM to RCF by pressing the “up” and “down” arrow keys simultaneously.

I want to convert from RPM to RCF. What is the radius of my MiniSpin plus Fixed-angle Rotor?

The MiniSpin plus can convert from RPM to RCF by pressing the “up” and “down” arrow keys simultaneously.

What is the maximum speed of the 5702?

The maximum speed for all rotors on the 5702 is 4,400 RPM. The RCF (g’s), which is determined by the rotor and the tube radius, ranges from 2,700-3,000.

Why can’t I get the rotor to tighten onto the 5702?

The rotor is not properly seated to the motor shaft. Follow the marking triangles on each side of the rotor nut. They indicate the direction of the groove on the underside of the rotor. Fit the rotor onto the motor shaft so the marking triangles point in the same direction as the motor shaft driver pin. Once fully seeded tighten the rotor nut by turning clockwise using the rotor key.

What does “At set rpm” on my 5702 R mean?

This will delay the beginning count down of the set time until the centrifuge has reached the selected rotation speed.

What is the conversion between the tube adapters and the vacutainer tube on the 5702?
BD Vacutainer Part # Dimensions (mm) Rotor Adapter Number
366401 (10 ml) 16 x 100 A-4-38 (round bkts) 022639269
A-4-38 (rect bkts) 022639366
F-35-30-17 No Adapter needed
A-8-17 No Adapter needed
366401 (7 ml) 13 x 100 A-4-38 (round bkts) 022639242
A-4-38 (rect bkts) 022639323
368071 (10 ml) 16 x 100 A-4-38 (round bkts) 022639269
A-4-38 (rect bkts) 022639366
F-35-30-17 No Adapter needed
A-8-17 No Adapter needed
368042 (10 ml) 16 x 100 A-4-38 (round bkts) 022639269
A-4-38 (rect bkts) 022639366
F-35-30-17 No Adapter needed
A-8-17 No Adapter needed

EXTRAN® is a registered trademark of E. Merck KGAA Limited.


Microcentrifuge Tubes

What are the minimum and maximum temperatures Eppendorf microcentrifuge tubes can withstand?

Eppendorf tubes™ are rated from –20°C to 100°C.

Do Eppendorf tubes contain any trace metals?

No. Eppendorf tubes are free of trace metals.

Does Eppendorf offer a siliconized tube?

No. Due to precision manufacturing processes, Eppendorf microcentrifuge tubes are often suitable for applications requiring siliconized tubes. Eppendorf tubes are manufactured with high precision injection molds and high quality pure polypropylene, resulting in a smooth uniform inner surface that minimizes sample retention. Please contact Customer Support at 800-645-3050 to request tube samples.

What is the maximum g-force Eppendorf microcentrifuge tubes are able to withstand?

Fully supported Eppendorf Flex-Tubes® and Eppendorf Safe-Lock Tubes can withstand g-forces up to 25,000 x g. In fact, Eppendorf Safe-Lock Tubes can withstand g-forces as high as 70,000 x g in a vertical ultracentrifuge rotor!

What are the dimensions of Eppendorf microcentrifuge tubes?
Tube description Dimensions (mm)
0.5 ml microcentrifuge tube 8 x 31
1.5 ml microcentrifuge tube 11 x 41
2.0 ml microcentrifuge tube 11 x 41
What quantity of Heparin-Li, Heparin-Li-F, or EDTA is coated on the anticoagulant-coated tubes?

Each microcentrifuge tube is coated with sufficient quantities of anticoagulant to achieve the following final concentrations of anticoagulant per ml of blood.

EDTA 2 mg EDTA / ml blood
Heparin-Li 30 U.I. / ml blood
Fluoride 2 mg / ml blood
What is the expected shelf life of anticoagulant-coated tubes?

The shelf life is unlimited as long as the tubes are packed in sealed bags and are not exposed to heat, sun, or humidity. The tubes should be stored at temperatures between 20°C and 30°C.

How do I know which type of anticoagulant should be used for my application?

EDTA is the anticoagulant of choice for all routine hematological investigations. Heparin Lithium is recommended blood gas analysis. Fluoride acts as a glycolysis inhibitor and is usually combined with an anticoagulant for the performance of glucose determination.

What protection do amber-colored Eppendorf microcentrifuge tubes provide across the photometric spectrum for light sensitive biological samples?

Amber-colored Eppendorf tubes offer reliable protection from light for nucleic acids or dyes. The following diagram shows a photometric differential measurement of light absorption in the range 240 nm to 700 nm between an amber-colored and a standard Safe-Lock microcentrifuge tube (wall thickness of both tubes: 0.8 mm).

In the range 240 nm to 500 nm, the difference in light extinction (DA) between the amber-colored and the standard Safe-Lock microcentrifuge tube is always greater than 2. This means a reduction of light radiation by a factor of 100. At 260 nm (the absorption maximum of nucleic acids), an extinction difference greater than 3 can be obtained, meaning that light radiation is reduced to less than one thousandth. Especially in the case of high-energy, short wavelengths, excellent light protection for sensitive samples is obtained with the amber-colored tubes.

Can Eppendorf tubes be autoclaved?

Yes. When in the open position, Eppendorf tubes can be autoclaved at 121°C for 20 minutes.

Can I sterilize Eppendorf microcentrifuge tubes with Ethylene Oxide?

Yes, However, please take necessary precautions when using this method.


Vacufuge Vacuum Concentrator

DISCLAIMER
To avoid risk of personal injury and/or damage to the Vacufuge, do not attempt to manually open the lid until all of the following criteria have been satisfied:
  • Unplug the Vacufuge
  • Turn the power switch off
  • Wait for the rotor to stop spinning

 

How do I open the lid in case of a power failure?

In the event of power failure, the ventilation valve will automatically open and the chamber will aerate to atmospheric pressure before the rotor stops spinning. Locate the pinhole on the right side of the housing, near the top rear corner. Insert a paper clip and the lid will open.

Why doesn’t the Eppendorf Vacufuge require a refrigerated or chemical trap?

The Vacufuge is designed to concentrate DNA, RNA, nucleotides, and proteins which are dissolved in alcohol or aqueous solutions. Up to 85% of these vapors are condensed in the supplied trap. Uncondensed alcohol and aqueous solvents are not hazardous to the lab environment, and therefore it is not necessary to use refrigerated or chemical traps.

A stainless steel dry ice trap is available for hazardous chemicals. (Brinkmann catalog number: 015509759.)

Do I need an in-line trap to protect the pump?

The Vacufuge uses a Teflon® pump that is resistant to most chemicals. The pump is made of Teflon, FFKM, and aluminum. These materials are chemically resistant to most chemicals therefore an in-line trap is not necessary.

What is the pumping capacity and absolute pressure of the supplied vacuum pump?

The pumping capacity is 30 L/min and the absolute pressure is 15 mBar. A Teflon vacuum pump with a pumping capacity of 64 L/min or an absolute pressure of 1 mBar is also available.

Can the basic unit without pump be upgraded to a complete system?

Yes. An easy-to-install upgrade kit is available. (Brinkmann catalog numbers: 022820206 for 115 V Model, and 022820222 for 200 V Model.)

Can I connect the supplied built-in vacuum pump to other systems (i.e. gel dryer)?

Yes and no. The built-in vacuum pump cannot be adapted for use with other systems, however, you can purchase a Vacufuge basic unit without pump along with a Büchi® Model V-500 Teflon Vacuum Pump for this purpose.

How long will it take for me to dry my sample?

The following table offers typical drying times for common solvents.

Rotor loading Temp. (°C ) Ventilation mode Time required
24 tubes, with 10 �l of 70 % ethanol 60 Alcohol 4.5 min.
96 tubes, with 10 �l of 70 % alcohol 60 Alcohol 6 min.
24 tubes, with 1 ml of distilled water 60 Aqueous 5 hr. 10 min.
24 tubes, with 1 ml of urine Ambient Aqueous 5 hr. 30 min.
24 tubes, with 1 ml of dichloro-methane RT Alcohol 20 min.
24 tubes, with 1 ml of dichloro-methane/isopropanol (80:20) RT Alcohol 40 min.
24 tubes, with 1 ml of hexane/ethyl acetate (80:20) RT Alcohol 18 min.

Teflon® is a registered trademark of E.I. du Pont de Nemours and Company.

Centrifuges: General Care Tips

Centrifuges: General Care Tips

  • Some paperwork must be done, before arriving at the details of centrifuge care. First, there are guides—your centrifuge came with one, also it carries a portion on maintenance and cleaning, and quite possibly a listing of chemicals/cleaners/disinfectants that are safe to utilize, along with a list of those that are not. A duplicate is likely accessible on the internet, and ought to be downloaded and printed in case the manual is lost. It’s a good idea to read it, and keep it handy, not only during initial runs but during the first cleanings also. A maintenance log should be kept so that appropriate maintenance could be confirmed to come full circle. This is especially significant in warranty claims.
  • The centrifugal field which accelerates the separation process also uses big forces on the rotor material. The centrifuge is severely damaged as well if a rotor fails. For this reason, some easy precautions ought to be observed. Rotors were created to be run up to their maximum speed using a load of a specific weight. One should never attempt to run a rotor at a rate higher than the one designated by its manufacturer. Also, if high density solutions (greater than 1.2 g/mL, for instance) are used, the run speed must be reduced to prevent undue stress on the rotor. Consult your instruction manual for precise directions.
  • Any glass fragments should be taken off the buckets, adapters, rubber liners, and rotor chamber before the following run is made. It must be cleaned up also, in case you find gray dust, which results from sandblasting by glass particles of the rotor chamber. You clean the chamber between each run to make sure this dust is discharged from the centrifuge, and need to make several dry runs without samples.
  • Consult your manual to be certain they are compatible with the various plastics and metals including the centrifuge, the rotor, the tubes, and other accessories if you intend to centrifuge any uncommon solvents or solutions. These same precautions must be detected with any solvents used for sterilization purposes.
  • Portions of the mist will likely be fine enough to form a relatively secure aerosol that will tend to be dispersed throughout the lab. Such spills should be thoroughly cleaned up before running the centrifuge.
  • Blood should be handled with respect for this possibility during all lab exploitation, including centrifugation.
  • From time to time, you’ll have questions about the actual operation as well as upkeep of your centrifuge. The instruction manual provided with each device was created to answer these questions. It kept handy for future reference, and ought to be read before making your first run.
  • (It is also often recommended to get staff assigned to this and other jobs.) While sometimes inconvenient, adhering to a regular program is cost-efficient and saves time in the long run.
  • For hazardous spills, your laboratory or association is going to have protocol that could contain everything from kinds of protective gear and merchandises, such as spill kits, to be used, to reporting and security procedures. It truly is essential that they are followed and that these are understood. It is often true that shortcuts, because they skip a step or detail, wind up taking more time later. Understand the types of samples being run in the laboratory; keep this info current, notably in a shared lab. This will definitely help ascertain whether protocols or any unique merchandises are needed for cleaning. The manual will probably be the very best guide to the goods and procedures to use for cleaning every one of these.
  • Centrifuge maintenance, as the name says, is to be understood as an everyday tool to maintain security and best condition of laboratory devices. To follow cleaning and care recommendations is tremendously significant in the event of pollution with salts and compounds. In case of long term exposure aggressive substances can develop corrosive areas on rotors and rotor-buckets, which might always grow and form little holes if ignored. This farther facilitates corrosion procedures as protective coatings are missing on those areas, if little scratches and cracks are present on the rotor surface. In ways that makes centrifugation procedures unsafe, pails or rotors could be damaged in case of advanced corrosion. In worst case, damaged rotors can even cause a crash. Yet the centrifuge and rotor themselves might be destroyed.
    Top quality aluminum rotors and rotor -buckets are usually protected against corrosion caused by commonly-used lab compounds, for example by way of an anodized coating. However competitive compounds can nevertheless damage the gear! Such chemicals include concentrated acids concentrated and light alkalis, solutions including copper ions mercury ions and other heavy metal ions, chlorinated hydrocarbons, and concentrated saline solutions. Generally steel rotors are protected against corrosion by the use of coatings too, but of course additionally they need constant care.
  • Centrifuges Remove pails and rotor from the centrifuge. For refrigerated centrifuges: Leave centrifuge lid defrost and open the ice on the rotor chamber surface. If your centrifuge includes a water collection tray, empty
  • We recommend checking the rotors and rotor bores visually for corrosion and deposit on a monthly or weekly basis. Plates or damaged tubes should not be centrifuged. Significant information regarding care and cleaning can not only be uncovered in the operating manual of your centrifuge, but in addition in this paper. If unsure concerning the cleaning or decontamination methods of the centrifuge or rotor, one
    should contact the manufacturer and follow his recommendation. An authorized technician should be reached, if in doubt whether a rotor remains safe.
    And clean it, then wipe the rotor chamber with a damp cloth. The outside of the centrifuge and also the rotor chamber should be cleaned frequently with a moist cloth and neutral detergents. Change off the centrifuge, before you do so.
  • When there’s broken glass: Retrieve the bigger broken glass with forceps, remove little and powder shards with a damp lint-free fabric. Consume blood with gauze or paper towel and afterwards, seal the material in a biohazard bag for safe disposal. Wipe the parts that are contaminated with
    neutral agents for cleaning and disinfection (e.g. diluted neutral alcohol-based disinfectant or 70% isopropanol mixture). Use a soaked gauze or lint-free fabric.
    If necessary, remove still contaminated pail caps out of centrifuge to decontaminate places that are difficult to reach, rotor lid, pails, and rotor. With distilled water the equipment ought to be wiped after decontamination.
  • Use inert cleaning solutions (alcohol or alcohol-based disinfectant) along with a soft cloth to clean your rotors and accessories. Wipe contaminated parts with neutral agents for cleaning and disinfection (e.g. diluted inert alcoholbased disinfectant or 70% isopropanol mixture). Use a soaked gauze or lint-free material. In case of some competitive liquid on your own centrifuge gear, please clean it instantaneously. When there is stubborn spot, clean with a plastic scrub pad and rinse equipment with distilled water.
    Liquid dry thoroughly with a soft cleaning cloth, and could flow into rotor cavities. We strongly urge cleaning buckets and rotors immediately, as the surface wills corrode. When there’s a need to wash the rotor’s boreholes or tube cavities, utilize a stiff test-tube brush that’s end bristles along with a nonmetallic tip. For swing-bucket rotors, ensure the grooves where the buckets are fitted are free of pollution. Take care to make sure that the pails can still swing freely.
  • Alternatively dry rotors, rotor accessories and boreholes with absorbent towels. To keep aerosol-tight hats/caps and seals from becoming worn out/damaged, shop lids/caps separately from the bucket/rotor.
  • To protect human beings from contamination from pathogens or samples, sterilization of rotors and accessories may be desired. Sterilization is a term referred to a process which removes all types of microbial life, including transmissible agents such as bacteria, viruses, fungi, spore forms etc.
    For autoclaving at 121 °C, a number of rotors in the marketplace have been analyzed. Particularly the aluminum made fixed-angle and swing out rotors have been vigorously tested and approved within this setting. They possess a special anodized coating which protects the metal from corrosion effects that are deeper. Steel made rotors are usually of limited suitability for autoclaving. Please ask the manufacturer about autoclaving chances in case you are in doubt about your rotor.
  • To realize an excellent chemical resistance against sodium hypochlorite and phenol, acetonitrile, DMSO, acetone, trichloroacetic acid, acetic acid, Eppendorf offers another type of aluminum fixed-angle rotor with a PTFE coating that is special. This coating is applied on top of the actual anodic coating. Autoclaving (121 °C, 20 min.) this rotor sort
    even improves the already enhanced chemical resistance as a result of material properties of the coating.

Autoclaves: General Care Tips

Autoclaves: General Care Tips

Weekly Autoclave Care

1. CLEAN EXTERNAL SURFACES
(a) Wipe with a soft dry cloth and occasionally with a damp
Material and mild soap or detergent.
2. CLEAN INTERNAL SURFACES
(a) Drain water from reservoir using drain tube located on
front of unit.
(b) Clean trays, door gasket, metal surfaces and inside of
chamber with Speed-Clean Sterilizer Cleanser and distilled
water. Scrutinize door gasket for damage that could prevent appropriate
sealing.
(c) Refill reservoir with tidy, distilled water.
3. DISINFECTING: (Refer to Setup/Operation guide)

Monthly:
1. FLUSH SYSTEM
(a) Drain reservoir and fill with clean distilled water. Add 1 oz.
of Speed-Clean Sterilizer Cleanser to a cool chamber (A).
(b) Run one POUCHES cycle. Press STOP button
when Drying part of cycle begins.

(c) Drain reservoir and refill reservoir with tidy, distilled
water.
(d) Rinse by running one UNWRAPPED cycle.
(e) Drain reservoir and allow sterilizer to cool to room
temperature.
(f) Remove door gasket, dam gasket and gasket ring. Clean
with Speed-Clean Sterilizer Cleaner and clean, distilled
water. A little stiff bristle brush will assist process. After
cleaning gaskets, inspect for damage, shrinking, or
swelling and replace if necessary. Press
gasket and ring into the channel and
reinstall dam gasket.
(g) Remove trays, tray stand, and tray
plate. Pressing downward on top
Group of tray rack, pull upward on
end of tray plate and slide
assembly outside of chamber.

(h) Locate chamber filters (B) on bottom and back of chamber.
Grab filter and pull out while twisting slightly. (If required,
a pair of pliers could be used). Filter may be cleaned with
Speed-Clean Sterilizer Cleaner and clean, distilled water. A
Little stiff bristle brush or ultrasonic cleaner might be helpful to
remove foreign objects from filter surface. Rinse filter with
clean, distilled water. NOTE – If cleaning approaches don’t
Essentially clean the filter, replacing may be necessary.
Reinstall filters by pressing inwards and twisting slightly.
(i) Wipe off trays, tray rack, and tray plate. Reinstall assembly
by placing rear edge of tray plate in chamber. Pushing
downward in addition to tray stand, slowly push assembly into
chamber.
(j) Fill the reservoir with clean, distilled water. Sterilizer is
now prepared to be used.
2. PRESSURE RELIEF VALVE CHECK
Refer to Installation and Operation Manual for this
Process

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