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Eppendorf Centrifuge F.A.Q

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How do I open the centrifuge lid in case of a power failure?
Eppendorf Microcentrifuge 5415 C 
Turn off the main power switch, unplug the centrifuge, and wait until the rotor stops spinning. Locate the pinhole on the left side of the housing, near the top right corner. Insert a paper clip and the lid will open.
Eppendorf Microcentrifuge 5415 D 
Turn off the main power switch, unplug the centrifuge, and wait until the rotor stops spinning. Locate the white plastic knob near the front right corner of the bottom base plate. The knob is attached to the emergency lid release mechanism by a string. Pull on the plastic knob while pushing down on the centrifuge lid and the lid will open.
Eppendorf Microcentrifuge 5415 R 
Turn off the main power switch, unplug the centrifuge, and wait until the rotor stops spinning. Remove the white cap near the front of the right side of the housing. Insert the rotor tool and turn clockwise while pushing down on the lid. The lid will open.
Eppendorf Microcentrifuge 5417 C and 5417 R 
Turn off the main power switch, unplug the centrifuge, and wait until the rotor stops spinning. Locate the plastic knob near the front left corner of the bottom base plate. The knob is attached to the emergency lid release mechanism by a string. Pull on the knob and the lid will open.
Eppendorf MiniSpin® and MiniSpin plus Personal Micro Centrifuges 
Turn off the main power switch, unplug the centrifuge, and wait until the rotor stops spinning. Insert a pen into the opening near the front right corner of the bottom plate of the centrifuge and move the disc counterclockwise until the lid opens.
Eppendorf Multipurpose Centrifuges 5804, 5804 R, 5810, and 5810 R 
Turn off the main power switch, unplug the centrifuge, and wait until the rotor stops spinning. Remove the cap located on the front housing, near the bottom center. Insert the rotor tool into the hole and turn counterclockwise until lid opens.
Eppendorf General Purpose Centrifuge 5702
Turn off the main power switch, unplug the centrifuge, and wait until the rotor stops spinning. Locate the plastic cap near the front right suction foot on the bottom base plate. The cap is attached to the emergency lid release mechanism by a string. Pull on the cap and the lid will open.
I have just received an Eppendorf Microcentrifuge 5417 C (or 5417 R) and a standard 30-place Fixed-angle Rotor. How do I install the rotor seal supplied with the rotor?

A seal has been installed by the manufacturer, therefore you do not need to install the spare seal provided.

I have an Eppendorf Multipurpose Centrifuge 5804 (5804 R, 5810, or 5810 R) and just changed my rotor from a Swing-bucket Rotor to a 6 x 85 ml Fixed-angle Rotor. Why does the display still indicate the maximum speed of the Swing-bucket Rotor? (In other words, the centrifuge is not recognizing the rotor.)

Eppendorf Multipurpose Centrifuges feature automatic rotor recognition. When you press Start, the centrifuge will ramp up to 200 rpm, display the new maximum speed, and then come to a stop. For manual rotor recognition, hold down the Start button and spin the rotor until the display changes to the appropriate maximum speed.

I just received a new Horizontal Drum Rotor or Deep-well Rotor for my Multipurpose Centrifuge 5804 (5804 R, 5810, or 5810 R). Why is my centrifuge not recognizing these new rotors?

The new rotors are compatible with software versions greater than 2.1. Check the software version by turning on the centrifuge and reading the display. New software EPROM’s are included with each new rotor and should be installed by an authorized Brinkmann service representative. Please contact our service department to schedule an appointment for installation of the new EPROM.

I just purchased an Eppendorf Microcentrifuge 5415 D and I did not receive a rotor tool.

The rotor tool is packed in the Styrofoam shipping insert.

I did not receive a rotor with my Eppendorf Microcentrifuge 5415 D.

For user convenience, Model 5415 D is sold either with or without rotor. The catalog numbers reflecting both options are listed below:

022621203Microcentrifuge 5415 D, with 24-place 1.5–2.0 ml rotor
022621211Microcentrifuge 5415 D, without rotor

Please check the catalog number of the version you’ve received and compare it to your purchase order. If you ordered catalog number 022621203 and did not receive a rotor, please contact our Customer Support department.

How often should I lubricate my centrifuge motor shaft?

Every time you change or install a new rotor.

Why can’t my Refrigerated Multipurpose Centrifuge 5804 R (or 5810 R) maintain 4°C at maximum speed?

The Multipurpose Centrifuges 5804 R and 5810 R can maintain 4°C at maximum speed with all rotors except the 6 x 85 ml Fixed-angle Rotor (022627207). If 4°C cannot be maintained at maximum speed with rotors other than the 6 x 85 ml Fixed-angle Rotor, please contact Brinkmann Instruments Services.

The following specifications apply to the 6 x 85 ml Fixed-angle Rotor only:

  • Models 5804 R and 5810 R can maintain 4°C with this rotor at 10,500 rpm
  • Model 5804 R can maintain 6°C at maximum speed
  • Model 5810 R can maintain 12.5°C at maximum speed
Why can’t my Refrigerated Centrifuge Model 5417 R, 5804 R, or 5810 R maintain 4°C overnight?

The refrigeration system of Eppendorf Refrigerated Centrifuge Models 5417 R, 5804 R, and 5810 R is designed to shutdown after six hours from the last time of use. This feature conserves energy, minimizes condensation buildup, and prolongs the lifetime of the centrifuge. Although it is not recommended, this feature may be switched off. Please contact Brinkmann Instruments Services for instructions.

Can I spin different tube sizes in my Multipurpose Centrifuge Swing-bucket Rotor?

Yes. Tubes of different volumes can be loaded in adjacent buckets, but not in opposite buckets. Please refer to the Operation Manual for loading Swing-bucket Rotors.

What is the warranty of my centrifuge?

Models 5415 D, 5415 R, 5417 C, 5417 R, 5702, 5702 R, 5804, 5804 R, 5810, 5810 R, MiniSpin, and MiniSpin plus all feature a two-year warranty. Models 5415 C and 5410 have a one-year warranty.

How should I clean my Centrifuge Rotor?

Rotors should be cleaned with neutral cleaning agents such as EXTRAN® neutral or isopropanol. Use neutral decontaminants such as RAD-Con and Radiacwash for radioactive decontamination. Do not use alkaline-cleaning agents such as Count-off.

I want to convert from RPM to RCF. What is the radius of my Model 5417C/R 30-place Fixed-angle Rotor?

Both models 5417C and 5417R can convert from RPM to RCF by pressing the “up” and “down” arrow keys simultaneously.

I want to convert from RPM to RCF. What is the radius of my MiniSpin plus Fixed-angle Rotor?

The MiniSpin plus can convert from RPM to RCF by pressing the “up” and “down” arrow keys simultaneously.

What is the maximum speed of the 5702?

The maximum speed for all rotors on the 5702 is 4,400 RPM. The RCF (g’s), which is determined by the rotor and the tube radius, ranges from 2,700-3,000.

Why can’t I get the rotor to tighten onto the 5702?

The rotor is not properly seated to the motor shaft. Follow the marking triangles on each side of the rotor nut. They indicate the direction of the groove on the underside of the rotor. Fit the rotor onto the motor shaft so the marking triangles point in the same direction as the motor shaft driver pin. Once fully seeded tighten the rotor nut by turning clockwise using the rotor key.

What does “At set rpm” on my 5702 R mean?

This will delay the beginning count down of the set time until the centrifuge has reached the selected rotation speed.

What is the conversion between the tube adapters and the vacutainer tube on the 5702?
BD Vacutainer Part #Dimensions (mm)RotorAdapter Number
366401 (10 ml)16 x 100A-4-38 (round bkts)022639269
A-4-38 (rect bkts)022639366
F-35-30-17No Adapter needed
A-8-17No Adapter needed
366401 (7 ml)13 x 100A-4-38 (round bkts)022639242
A-4-38 (rect bkts)022639323
368071 (10 ml)16 x 100A-4-38 (round bkts)022639269
A-4-38 (rect bkts)022639366
F-35-30-17No Adapter needed
A-8-17No Adapter needed
368042 (10 ml)16 x 100A-4-38 (round bkts)022639269
A-4-38 (rect bkts)022639366
F-35-30-17No Adapter needed
A-8-17No Adapter needed

EXTRAN® is a registered trademark of E. Merck KGAA Limited.


Microcentrifuge Tubes

What are the minimum and maximum temperatures Eppendorf microcentrifuge tubes can withstand?

Eppendorf tubes™ are rated from –20°C to 100°C.

Do Eppendorf tubes contain any trace metals?

No. Eppendorf tubes are free of trace metals.

Does Eppendorf offer a siliconized tube?

No. Due to precision manufacturing processes, Eppendorf microcentrifuge tubes are often suitable for applications requiring siliconized tubes. Eppendorf tubes are manufactured with high precision injection molds and high quality pure polypropylene, resulting in a smooth uniform inner surface that minimizes sample retention. Please contact Customer Support at 800-645-3050 to request tube samples.

What is the maximum g-force Eppendorf microcentrifuge tubes are able to withstand?

Fully supported Eppendorf Flex-Tubes® and Eppendorf Safe-Lock Tubes can withstand g-forces up to 25,000 x g. In fact, Eppendorf Safe-Lock Tubes can withstand g-forces as high as 70,000 x g in a vertical ultracentrifuge rotor!

What are the dimensions of Eppendorf microcentrifuge tubes?
Tube descriptionDimensions (mm)
0.5 ml microcentrifuge tube8 x 31
1.5 ml microcentrifuge tube11 x 41
2.0 ml microcentrifuge tube11 x 41
What quantity of Heparin-Li, Heparin-Li-F, or EDTA is coated on the anticoagulant-coated tubes?

Each microcentrifuge tube is coated with sufficient quantities of anticoagulant to achieve the following final concentrations of anticoagulant per ml of blood.

EDTA2 mg EDTA / ml blood
Heparin-Li30 U.I. / ml blood
Fluoride2 mg / ml blood
What is the expected shelf life of anticoagulant-coated tubes?

The shelf life is unlimited as long as the tubes are packed in sealed bags and are not exposed to heat, sun, or humidity. The tubes should be stored at temperatures between 20°C and 30°C.

How do I know which type of anticoagulant should be used for my application?

EDTA is the anticoagulant of choice for all routine hematological investigations. Heparin Lithium is recommended blood gas analysis. Fluoride acts as a glycolysis inhibitor and is usually combined with an anticoagulant for the performance of glucose determination.

What protection do amber-colored Eppendorf microcentrifuge tubes provide across the photometric spectrum for light sensitive biological samples?

Amber-colored Eppendorf tubes offer reliable protection from light for nucleic acids or dyes. The following diagram shows a photometric differential measurement of light absorption in the range 240 nm to 700 nm between an amber-colored and a standard Safe-Lock microcentrifuge tube (wall thickness of both tubes: 0.8 mm).

In the range 240 nm to 500 nm, the difference in light extinction (DA) between the amber-colored and the standard Safe-Lock microcentrifuge tube is always greater than 2. This means a reduction of light radiation by a factor of 100. At 260 nm (the absorption maximum of nucleic acids), an extinction difference greater than 3 can be obtained, meaning that light radiation is reduced to less than one thousandth. Especially in the case of high-energy, short wavelengths, excellent light protection for sensitive samples is obtained with the amber-colored tubes.

Can Eppendorf tubes be autoclaved?

Yes. When in the open position, Eppendorf tubes can be autoclaved at 121°C for 20 minutes.

Can I sterilize Eppendorf microcentrifuge tubes with Ethylene Oxide?

Yes, However, please take necessary precautions when using this method.


Vacufuge Vacuum Concentrator

DISCLAIMER
To avoid risk of personal injury and/or damage to the Vacufuge, do not attempt to manually open the lid until all of the following criteria have been satisfied:
  • Unplug the Vacufuge
  • Turn the power switch off
  • Wait for the rotor to stop spinning

 

How do I open the lid in case of a power failure?

In the event of power failure, the ventilation valve will automatically open and the chamber will aerate to atmospheric pressure before the rotor stops spinning. Locate the pinhole on the right side of the housing, near the top rear corner. Insert a paper clip and the lid will open.

Why doesn’t the Eppendorf Vacufuge require a refrigerated or chemical trap?

The Vacufuge is designed to concentrate DNA, RNA, nucleotides, and proteins which are dissolved in alcohol or aqueous solutions. Up to 85% of these vapors are condensed in the supplied trap. Uncondensed alcohol and aqueous solvents are not hazardous to the lab environment, and therefore it is not necessary to use refrigerated or chemical traps.

A stainless steel dry ice trap is available for hazardous chemicals. (Brinkmann catalog number: 015509759.)

Do I need an in-line trap to protect the pump?

The Vacufuge uses a Teflon® pump that is resistant to most chemicals. The pump is made of Teflon, FFKM, and aluminum. These materials are chemically resistant to most chemicals therefore an in-line trap is not necessary.

What is the pumping capacity and absolute pressure of the supplied vacuum pump?

The pumping capacity is 30 L/min and the absolute pressure is 15 mBar. A Teflon vacuum pump with a pumping capacity of 64 L/min or an absolute pressure of 1 mBar is also available.

Can the basic unit without pump be upgraded to a complete system?

Yes. An easy-to-install upgrade kit is available. (Brinkmann catalog numbers: 022820206 for 115 V Model, and 022820222 for 200 V Model.)

Can I connect the supplied built-in vacuum pump to other systems (i.e. gel dryer)?

Yes and no. The built-in vacuum pump cannot be adapted for use with other systems, however, you can purchase a Vacufuge basic unit without pump along with a Büchi® Model V-500 Teflon Vacuum Pump for this purpose.

How long will it take for me to dry my sample?

The following table offers typical drying times for common solvents.

Rotor loadingTemp. (°C )Ventilation modeTime required
24 tubes, with 10 �l of 70 % ethanol60Alcohol4.5 min.
96 tubes, with 10 �l of 70 % alcohol60Alcohol6 min.
24 tubes, with 1 ml of distilled water60Aqueous5 hr. 10 min.
24 tubes, with 1 ml of urineAmbientAqueous5 hr. 30 min.
24 tubes, with 1 ml of dichloro-methaneRTAlcohol20 min.
24 tubes, with 1 ml of dichloro-methane/isopropanol (80:20)RTAlcohol40 min.
24 tubes, with 1 ml of hexane/ethyl acetate (80:20)RTAlcohol18 min.

Teflon® is a registered trademark of E.I. du Pont de Nemours and Company.

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