The Eppendorf IsoTherm Systems are designed to cool samples effectively and consistently over many hours at 0°C or at –21°C. The White IsoTherm System is designed for 0°C and the Blue IsoTherm System is designed for –21°C. The Systems consist of the following components: the IsoPack (cold pack), the IsoRack (transparent storage rack), and the IsoSafe (insulated transport box).
How long do IsoPacks maintain their temperature?
White IsoTherm System: IsoPacks that are seated in IsoRacks and sealed in the insulating IsoSafe can maintain a temperature of 0°C up to six hours. If the IsoPacks are contained in an opened IsoSafe, then a temperature of 0°C can be maintained for approximately 4 hours. If IsoPacks/IsoRacks are not placed in an IsoSafe, then 0°C can be maintained for 1.5 to 3 hours. Blue IsoTherm System: IsoPacks that are seated in IsoRacks and sealed in the insulating IsoSafe can maintain a temperature of –21°C for over 3 hours. If the IsoPacks are contained in an opened IsoSafe, then a temperature of –21°C can be maintained for 1 to 2 hours. If IsoPacks/IsoRacks are not placed in an IsoSafe, then –21°C can be maintained for 0.5 to 1 hour.
At what temperatures can the IsoTherm Rack be stored?
The IsoTherm Rack can be stored at temperatures as low as –200°C.
What are IsoRacks made of?
IsoRacks are made of polyethylene and filled with a non-toxic liquid.
What are the IsoPack’s dimensions? What are the IsoRack’s dimensions?
Dimensions (W x D x H)
IsoPack for 0.5 ml tubes
4.7 x 3.0 x 0.9 in./12 x 7.7 x 2.3 cm
IsoRack for 0.5 ml tubes, with lid
5.0 x 3.4 x 1.9 in./12.7 x 8.6 x 4.9 cm
IsoPack for 1.5/2.0 ml tubes
4.8 x 3.1 x 1.5 in./12.2 x 8 x 3.9 cm
IsoRack for 1.5 and 2.0 ml tubes, with lid
5.0 x 3.4 x 2.5 in./12.7 x 8.6 x 6.4 cm
Are IsoPacks resistant to sodium hypochloride (NaOCl)?
Sodium Hypochloride (NaOCl) will attack the aluminum foil on both the blue and the white IsoPacks to some extent. Over a period of eight hours or more, this may lead to damage. The IsoPack’s polyethylene housing is chemically resistant to NaOCl. Even if a leak occurs, the liquid in the IsoPack is non-toxic.
Back to top
Why can’t I set the temperature higher than 70°C with the block for microplates (Brinkmann Catalog # 022670565)?
Many microplates are made of polystyrene, which becomes soft at 80°C. As a safety precaution, the Eppendorf Thermomixer R will not exceed temperatures of 70°C when using the block for microplates. If you are using polypropylene or Teflon® plates that are rated to 99°C, you may disable this safety feature.
To avoid risk of personal injury and damage to the Thermomixer, please be sure that when disabling this feature, only microplates and deep-well plates rated to 99°C are incubated.
In order to disable this safety feature, you must deactivate the block recognition system. Please follow the procedure outlined below.
Turn power on and set the speed to 500 rpm
Turn the power off
Turn the power on while holding the Temperature down arrow key; the display will indicate “service ON”
Scroll through the menu by pressing the Temperature up arrow key until the display indicates “Block ID”
To turn the block recognition off press the Mix up arrow key. To turn the block recognition on, press the Start/Stop button.
Do I need to fill the thermal block holes with water?
No. Eppendorf Thermomixer R takes advantage of Peltier technology, therefore cooling or heating fluid is not necessary. Filling the thermal block with water will damage the Thermomixer.
I have an older Eppendorf Thermomixer Model 5436. Can I use the Model 5436 thermal block with the Thermomixer R?
No. The model 5436 thermal block is not compatible with the Thermomixer R.
I need to cool my samples to 4°C. Can I run the Thermomixer R in a cold room to maintain 4°C?
Yes and no. The Eppendorf Thermomixer can be operated in a cold room, however, precise temperature control at 4°C is not guaranteed.