Beckman Coulter CEQ-8000 Analyzer


Condition: Refurbished and Tested to Manufacturer Spec
Warranty: 90-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 1 to 3 Business Days

1 in stock

Description

The Beckman Coulter CEQ-8000 Analyzer is a fully automated genetic analysis system that helps to find the base sequence and fragment length of DNA samples prepared with Beckman Coulter dye-labeled reagents. Base sequence analysis is performed by processing samples using four-color, dye-labeled terminator chemistry kits. Dye-labeled primers are used for performing fragment length analysis. The CEQ-8000 analyzer has the capacity to accept 96 four-color samples in a microplate. The automatic denaturation of DNA fragments followed by separation is done using capillary electrophoresis. Separation gel gets automatically replaced in the eight capillaries after each run. An easily replaceable cartridge provides the separation gel needed for a full microplate.

The CEQ-8000 Analyzer carries out detection with the means of laser-induced fluorescence in four spectral channels. High-quality base sequences or fragment lists are produced after separation by automatic processing of the raw data generated by each of the eight capillaries. The data in raw and analyzed form gets stored in a database and can be easily exported in compatible file formats, used commonly for analysis applications. Some of the useful hardware features of the CEQ-8000 are capillary temperature control, easily accessible arrays, sample transport and plate holders, and status indicator lights. The analyzer comes with pre-loaded software that helps in managing the system and carrying out analysis. The software offers the different modules to carry out varied tasks – Sample Setup Module, Run Module, Sequence Analysis Module, CEQuence Investigator Module, Fragment Analysis Module and Data Manager Module.

Manual: Beckman Coulter CEQ-8000 Analyzer Manual

Specifications


Consumable Items Required for Sequence Analysis

Item P/N QTYa Description
DTCS Kit (DNA 608000 1 Dye Terminator Cycle Sequencing Kit for 96 reactions. Includes:

• DNA polymerase

• CEQ Dye Terminators (ddUTP, ddGTP, ddCTP, ddATP)

• dNTP Mix Solution

• Sequencing Reaction Buffer

• pUC18 Control Template

• -47 Sequencing Primer

• Glycogen

• Mineral Oil

• Sample Loading Solution (SLS)

Sequencing RXN Kit)
or
DTCS Quick Start Kit 608120 1 Dye Terminator Cycle Sequencing Kit for 96 reactions in a single tube containing:

• DNA polymerase

• CEQ Dye Terminators (ddUTP, ddGTP, ddCTP, ddATP)

• dNTP Mix Solution

• Sequencing Reaction Buffer

• pUC18 Control Template

• -47 Sequencing Primer

• Glycogen

• Mineral Oil

• Sample Loading Solution (SLS)

Sequencing Test Samples 608070 1
CEQ Separation Gel LPA 1 608010 1 11.5 mL of gel in CEQ 8000 compatible container. Sufficient for 12 fills of the Capillary Array (96 sequencing runs).
CEQ Separation Buffer 608012 1 Each container has a screw top and pour tip. The container has enough buffer (30 mL) to fill a CEQ System 96-well, flat bottom Buffer Plate. (Each well being ¾ full.) 4/Pack
DNA Separation Capillary Array 33-75B 608087 1 Package of 100 flat-bottom polystyrene plates, nonsterile, without lids. Required for use as the CEQ 8000 Separation Buffer Plate.

Consumable Items Required for Sequence Analysis (Continued)

Item P/N QTYa Description
Buffer Plates 609844 Box Package of 100 flat-bottom polystyrene plates, nonsterile, without lids. Required for use as the CEQ Separation Buffer Plate.
Sample Plate 609801 Box Package of 25 V-bottom thermal cycler-compatible polypropylene plates with 200 µL volume capacity. Required for use as the CEQ 8000 sample plate. 25 Plates/Box

a. Contact your local Beckman Coulter representative for specific consumable material requirements based on your application and frequency of use.

Table 76 provides a list of the required consumable items for the Fragment Analysis system.

Required Consumable Materials for Fragment Analysis

Item P/N QTYa Description
DNA Separation Capillary Array 33-75B 608087 1 Eight capillaries, 75 µm i.d., 33 cm long, 200 o.d. complete with electrode block and detector array fitting. Ready for installation into CEQ 8000.
CEQ Separation Gel LPA 1 608010 1 11.5 mL of gel in CEQ 8000 compatible container. Sufficient for 12 fills of the Capillary Array (96 sequencing runs).
CEQ Separation Buffer 608012 1 Each container has a screw top and pour tip. The container has enough buffer (30 mL) to fill a CEQ System 96-well, flat bottom Buffer Plate. (Each well being ¾ full.) 4/Pack
CEQ DNA Size Standard Kit – 80 608395 1 Contains fragments of 13 and 80 nucleotides designed to accomodate a wide range of sizes for multiplexed and poolplexed SNP fragments.
CEQ DNA Size Standard Kit – 400 608098 1 DNA size standard for analysis of fragments up to 400 nucleotides. Includes:

• Mineral Oil

• DNA fragments of the following sizes labeled with CEQ WellRED fluorescent dye: 60, 70, 80, 90, 100, 120, 140, 160, 180, 190, 200, 220, 240, 260, 280, 300, 320, 340, 360, 380, 400, and 420 nucleotides

Sufficient for 96 fragment analysis separations.

Required Consumable Materials for Fragment Analysis

Item P/N QTYa Description
CEQ DNA Size Standard Kit – 600 608095 1 DNA size standard for analysis of fragments up to 600 nucleotides. Includes:

• Mineral Oil

• DNA fragments of the following sizes labeled with CEQ WellRED fluorescent dye: 60, 70, 80, 90, 100, 120, 140, 160, 180, 190, 200, 220, 240, 260, 280, 300, 320, 340, 360, 380, 400, 420, 440, 460, 480, 500, 520, 540, 560, 580, 600, 620, and 640 nucleotides

Sufficient for 96 fragment analysis separations.

CEQ SNP-Primer Extension Kit. 390280 1 Includes:

• DNA Polymerase

• CEQ dye-labeled terminators (ddUTP, ddGTP, ddCTP, ddATP)

• Reaction buffer

• Mineral oil

Sufficient for 96 reactions.

CEQ Sample Loading Solution (SLS) 608082 1 Sample Loading Solution (SLS), 6.0 mL
Buffer Plates 609844 Box Package of 100 flat-bottom polystyrene plates, nonsterile, without lids. Required for use as the CEQ Separation Buffer Plate.
Sample Plate 609801 Box Package of 25 V-bottom thermal cycler-compatible polypropylene plates with 200 µL volume capacity. Required for use as the CEQ 8000 sample plate. 25 Plates/Box.

 

 

Warranty Information


Laboratory Controls offers a best in the business full 90 day warranty on all refurbished items. This warranty covers your part against any material and or craftsmanship issues within one full year from purchase. Laboratory Controls will repair or replace your part at no charge. Please contact us for further information if you are in need of a repair.

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