Eppendorf 5331 MasterCycler Gradient Thermal Cycler

Condition: Refurbished and Tested to Manufacturer Spec
Warranty: 90-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 1 to 3 Business Days


Manual: Eppendorf 5331 MasterCycler Gradient Thermal Cycler

Overview of Eppendorf 5331 MasterCycler Gradient Thermal Cycler

The Eppendorf Mastercycler and Mastercycler gradient are PCR devices for all molecular biological and
biochemical laboratories in the field of basic research or routine diagnostics.

Temperature control is carried out with Peltier elements, which enable extremely rapid changes in temperature of up to 3 °C per second. This allows the temperature of the samples to be controlled extremely rapidly between 4 °C and 99 °C.

A special “tube control” mode has been developed for temperature-controlling samples in various different tubes with various different filling volumes.

The thermoblock has been constructed as a universal block which can accommodate microtiter plates in the 8 x 12 format, 96 Eppendorf PCR test tubes of 0.2 ml, 77 thin-walled Eppendorf PCR test tubes of 0.5 ml or other suitable tubes – without the block having to be exchanged.


Sample capacity: 1 PCR plate 96 or 96 0.2 ml PCR tubes or 77 0.5 ml PCR tubes, thin-walled
Temperature range: 4 to 99 °C
Temperature uniformity across the block: 95 °C ± 1.0 °C / 20 to 72 °C ± 0.6 °C
Control accuracy: ± 0.2 °C
Temperature-control speeds: Heating rate: approx. 3 °C/s, measured on the block
Cooling rate: approx. 2 °C/s, measured on the block
Number of programs: max. 99
max. 10 on a personal card
Maximum number of cycles: 99
Dimensions: Width: 26 cm
Depth: 41 cm
Height: 27 cm
Weight: approx. 12.4 kg
Voltage/frequency: 230 V, 50–60 Hz / 115 V, 50–60 Hz / 100 V, 50–60 Hz
Power requirement: 500 W 500 W 500 W
Operating current: 2.6 A 5 A 6 A
Fuses: 2 x T 5 A, 250 V 2 x T 6.3 A, 250 V 2 x MT 8 A, 250 V
Overvoltage category: II
Pollution degree: 2
Contamination level: I
Operating conditions: 15 to 35 °C, 70 % rel. humidity
Storage conditions: –20 to 70 °C, 85 % rel. humidity


Eppendorf 5331 MasterCycler Gradient Thermal Cycler Manual

Warranty Information

Laboratory Controls offers a best in the business full 90 day warranty on all refurbished items. This warranty covers your part against any material and or craftsmanship issues within one full year from purchase. Laboratory Controls will repair or replace your part at no charge. Please contact us for further information if you are in need of a repair.

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