Eppendorf 5332 Mastercycler weighs 6.8 kg and has dimensions of 18 x 35 x 25 cm (W x D x H). It carries the sample capacity of 1 microtiter plate or 25 x 0.2 ml PCR tubes or 16 x 0.5 ml thin walled PCR tubes. Eppendorf 5332 offers the temperature range between 4 to 99°C. It features controlled accuracy as ± 0.2 K. The heating rate is approximately 3 K/s (measured on the block) and the cooling rate is around 2 K/s (measured on the block). Maximum 99 programs can be stored in the machine and maximum 10 programs can be stored on a personal card. Eppendorf 5332 comes in three versions on the basis of voltage and frequency required for the operation: (i) 230 V, 50–60 Hz, (ii) 115 V, 50–60 Hz, (iii) 100 V, 50–60 Hz. It has three fuses: 2x 5 A slow, 2x 3.15 A slow, 2x 3.15 A slow. It comes with 1 pouch of PCR tubes 0.2 ml (100 pcs.), and 1 pouch of PCR tubes 0.5 ml thin-walled (100 pcs).
To ensure the functioning of the device upon receiving the product you need to switch on the device which displays device type and software version. Check keypad, distribute at least 4 – 6 PCR test tubes over the complete block and check whether the heated lid locks in the corresponding position (0.2ml or 0.5ml). You are required to check locking of heated lid with twin tec plates as well. Set the temperature of heated lid to approx. 105 °C, connect the printer if necessary, activate printer, call up “Standard” program. Start a program, check printer protocol, and stop program after approximately 10 minutes.
To clean the Eppendorf 5332, disconnect mains plug, and allow the device (thermo block and heated lid) to cool down to room temperature. Use a damp cloth to clean the outside of the device with water and a mild cleaning solution. To clean the inner surface of the machine, disengage the heated lid. Clean the sample tube position in the thermo block by using a damp Q-Tip and remove broken test tubes if necessary.
|features||max no. of cycles 99|
|no. of programs 100 (plus unlimited cards)|
|selectable temp. gradient across block: no|
|mfr. no.||Eppendorf®, 5332 000.014 (European)|
|availability||not available in USA and Canada|
|measuring range||4-99 °C temp, accuracy: 0.2 °C|
|temp uniformity across block||0.6 °C, 20-72 °C|
|1.0 °C, 95 °C|
|W × D × H||18 cm × 35 cm × 25 cm|
|power consumption||300 W|