MJ Research PTC-200 Gradient Thermal Cycler


Condition: Refurbished and Tested to Manufacturer Spec
Warranty: 90-Day Full Parts and Labor (Click for Info)
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Description

MJ Research PTC-200 DNA Engine is an easy-to-use, reliable and compact thermal cycler. It features interchangeable sample blocks (the Alpha unit family) which can accommodate many kinds of tubes, micro-plates, and slides. Its dimensions are 24 x 35 x 17cm. MJ Research PTC-200 is equipped with hot Bonnet heated lid for oil-free cycling. It has inbuilt intuitive software with easy-to-read user interface for quick programming, editing, file management, and password protection.  It gives you a choice of calculated sample temperature control for highest accuracy and speed.

MJ Research PTC-200 features space-saving design for quick setup and easy transportation. It offers instant incubate feature for continuous-temperature incubations.  MJ Research PTC-200 DNA Engine provides networking for up to 15 machines which enables convenient remote operation and documentation of runs. It can save 200 typical programs in up to 12 individual folders.

MJ Research PTC-200 comes with a one DNA Engine base, one Alpha, two spare fuses, one power cord, one probe (if purchased), PTC-200 DNA Engine Operations Manual, product registration card (for US customers only), extended warranty application ( for US customers only), and DNA Engine Driver remote software request form. PTC-200 DNA Engine requires 100–240VAC, 50–60Hz, and a grounded outlet. It requires a constant supply of air that is 31˚C or cooler to remove heat from the Alpha unit’s heat sink. Air is taken in from the vents at the front, back, and bottom of the machine and exhausted from vents on both sides. To avoid overheating, performance problems, software error messages, and sudden shut downs of the machine, ensure that the air supply is adequate and air flow is unobstructed.

Features:

  • Single-bay cycler accommodates over ten different Alpha™ unit sample blocks, including independently controlled dual blocks
  • Temperature gradient capability employs dynamic ramping, guaranteeing consistent hold times across all sample wells
  • Upgradable to real-time quantitative PCR with the purchase of a Chromo4™ four-color real-time detector

All DNA Engine family thermal cyclers offer interchangeable sample blocks, unprecedented thermal performance, and operational controls that facilitate incorporation of these cyclers into a variety of laboratory environments, including automated settings.

Advantages of all DNA Engine® family thermal cyclers:

  • Sample Blocks
    • Interchangeable Alpha unit sample blocks are designed for frequent change; they can be swapped in seconds and seat many types of reaction vessels
    • Independently controlled dual blocks are available for running two different protocols simultaneously
    • Gradient capability is standard on all DNA Engine systems when fitted with 96-well Alpha units
    • Unique Peltier heat pumps are built in-house specifically for cycling; years of experience and proprietary technologies make MJ modules last longer than any other
    • Multizone feedback control utilizes multiple sensors combined with four “zones” for heating and cooling to optimize thermal profiles every 50 ms
  • Performance
    • Extraordinary well-to-well temperature uniformity of ±0.4°C within 30 sec of arrival at 90°C, (±0.5°C for dual-block Alpha units)
    • Superb thermal accuracy, average temparature across sample block within ±0.3°C of NIST standard at 90°C, (±0.4°C for dual-block Alpha units)
    • Thermal range of 0°C to 105°C (4°C to 100°C for Slide Chambers™ unit)
    • Rapid ramp rates of up to 3.0°C/sec
  • Operation
    • Two thermal control options: block control or calculated control
    • Choice of two heated-lid designs that accommodate many vessel types and various sealing options, allowing maximum flexibility in experimental designs—heated lid temperatures can be set with any DNA Engine instrument
      • Manual Hot Bonnet® heated lid with a unique thumbwheel for adjusting height and pressure
      • Moto Alpha™ units can be remotely commanded to open, close, and adjust height and pressure—alternatively, they may be opened and closed with the touch of a button for highly repeatable sealing

 

 

DNA ENGINE SPECIFICATIONS

Operational Specifications

Speed of ramping: Up to 3°C/sec
Programmable ramp rates: Yes
Method of heating/cooling: Peltier-Joule
Temperature control methods: Block or calculated
Thermal range: 0-105°C
Accuracy: ±0.3°C of programmed target at 90°C, NIST-traceable
Uniformity: ±0.4°C well-to-well within 30 sec of arrival at 90°C

Descriptive

# of Alpha unit bays: 1
Sample capacity: Depends on Alpha unit used
Display: 80-character LCD, 3 LEDS, audible alert
Licensed for PCR: Yes
Operating temperature: 4°C-32°C ambient
Operating humidity: 10%-90% humidity
Communication: IEEE-488 bi-directional general purpose interface bus,
9-pin RS-232 serial port for printer or remote use,
25-pin 8-bit parallel interface printer port
Electrical approval: IEC, CE
Input power: 100-240 VAC, 50-60 Hz, 850 W maximum
Power failure restore: Yes
Dimensions (W x D x H): 24 x 33 x 24 cm (base only)
Weight: 9 kg (chassis and Alpha™ unit)

Programming

Memory: 400 typical programs; up to 12 password-protected folders
Note: Specifications subject to change without notice.

 

GRADIENT SPECIFICATIONS (96-WELL ALPHA UNIT ONLY)
Accuracy: ±0.3°C of programmed target at end columns within 30 sec after gradient step timer starts
Uniformity: ±0.4°C well-to-well within column, within 30 sec of reaching target temperature
Calculator Accuracy: ±0.4°C of actual well temperature
Lowest Programmable Gradient Temp: 30°C
Highest Programmable Gradient Temp: 105°C
Temperature Differential Range: 1°C-24°C
Note: Specifications subject to change without notice.

 

 

Temperature gradient available on all DNA Engine family thermal cyclers

The MJ gradient feature is now standard on any new cycler within the DNA Engine family equipped with the 96-well Alpha unit, including the original DNA Engine cycler, the DNA Engine Tetrad® 2cycler, the DNA Engine Dyad® cycler, and the Dyad Disciple cycler.
In addition, the gradient feature is also standard on the DNA Engine Opticon® continuous fluorescence detection systems. For DNA Engine family instruments purchased prior to August 31, 1999 a simple upgrade is available. (For 96-well sample blocks only.)

Molecular biology labs routinely need to optimize incubation temperatures for thermal cycling reactions. Optimization is critical but not always easy to do. Even after calculating the Tm (melting temperature) of a primer, the annealing temperature often needs to be determined empirically, which involves repeating a reaction at many different temperatures. Similar time-consuming tests may also be used to optimize the denaturation temperature.

The MJ gradient feature allows you to perform optimization reactions in a single experiment by using a range of temperatures simultaneously. With the thermal gradient software, you can program a temperature gradient up to 24°C across a 96-well sample block. The gradient feature also works with calculated sample temperature mode, so you can generate fully optimized programs in a single step.

Advanced features make optimization easy.

Dynamic ramping means that the temperature gradient forms during ramping. All wells come to the designated temperature at the same time, making the incubation period consistent among all samples in the experiment.

This graph represents the superposition of thermal measurements taken from twelve columns in four different cyclers (there are actually 48 separate traces in the graph). Four cyclers were programmed to develop a 45-65°C gradient—the bottom traces represent the leftmost columns and the top traces the rightmost columns. This was done to demonstrate both the extraordinary thermal performance of the instruments, and the cycler-to-cycler reproducibility of the profiles. Note too the even spread of temperatures, which allows for the greatest quantity of useful data.

 

 

The twelve points across the graph represent the temperature values displayed for each column of the block, with the size of the point far smaller than the specified error (±0.4°C). Four traces from four cyclers have been superposed, showing the cycler-to-cycler consistency.

 

 

 

Methods: Both graphs incorporate data from 48 wells of each cycler, taken with NIST-traceable, laser-trimmed thermistors.

GRADIENT SPECIFICATIONS
Gradient accuracy: ±0.3°C of target for left and right columns, and ±0.4°C of predicted temperature of all columns, 30 seconds after the timer starts for the gradient step, NIST-traceable
Gradient column uniformity: ±0.4°C well-to-well within the column, within 30 seconds of reaching target temperature
Gradient calculator accuracy: ±0.4°C of actual column temperature
Lowest programmable temperature for gradient step: 30°C
Highest programmable temperature for gradient step: 105°C
Temperature differential range: 1-24°C

 

 

Since 1994, the DNA Engine family of thermal cyclers has used multiple zones of thermal control across its sample blocks to deliver remarkable well-to-well temperature uniformity. Sophisticated algorithms use readings from three sensors in the block to adjust up to four separately-controlled heating/cooling elements, quickly and effectively bringing the sample block to uniformity. This same technology is used to establish a highly reproducible temperature gradient across the sample block using software alone – unlike other gradient-enabled thermal cyclers, which require specialized hardware. The MJ gradient software is now a standard feature, offered at no extra cost, on all new DNA Engine, Tetrad™ 2, Dyad™, and Dyad Disciple™ cyclers, and the DNA Engine Opticon® continuous fluorescence detection system.

The Software Upgrade

  • All DNA Engine Dyad® thermal cyclers and DNA Engine Opticon continuous fluorescence detection systems have the gradient software already installed as a standard feature
  • All DNA Engine and DNA Engine Tetrad® cyclers purchased after August 31, 1999 also have the gradient software already installed
  • DNA Engine cyclers purchased between January 1, 1999 and August 31, 1999 may be upgraded free of charge, using a CD-ROM field upgrade package and your Power Mac or PC (Win 95/98)
  • DNA Engine cyclers purchased before January 1, 1999, and DNA Engine Tetrad cyclers purchased before August 31, 1999 may be sent to Bio-Rad laboratories for a low-cost upgrade. Please call (888) 735-8437 (888-PELTIER) for details.
MULTIZONE SPECIFICATIONS
Gradient accuracy: ±0.3°C of target for left and right columns, and ±0.4°C of predicted temperature of all columns, 30 sec after the timer starts for the gradient step, NIST-traceable
Gradient column uniformity: ±0.4°C well-to-well within the column, within 30 sec of reaching target temperature
Gradient calculator accuracy: ±0.4°C of actual column temperature
Lowest programmable temperature for gradient step: 30°C
Highest programmable temperature for gradient step: 105°C
Temperature differential range: 1-24°C
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